Indian Journal Of Animal Health

Published by

West Bengal Veterinary Association
NAAS SCORE - 4.08

Last Issue
VOL. 57, NO.2 2 DECEMBER, 2018
 
 

Review Article

INTERACTIONS  OF  PLANT  BIOACTIVES  WITH  NUTRIENT  TRANSPORT  SYSTEMS  IN

GUT  OF  LIVESTOCK

-----------------------------------------------A. K. PATRA

Department of Animal Nutrition

West Bengal University of Animal and Fishery Sciences

37 K. B. Sarani, Belgachia, Kolkata- 700 037, India

Research on natural plant secondary compounds (PSC) has gained widespread impetus for use as feed additives to improve production performance, welfare and health of livestock and poultry due to their antimicrobial, antioxidant, immunostimulatory and other beneficial biological effects. Moreover, various PSC are usually present in regular diets of animals and humans. Dietary PSC may regulate absorption of nutrients, minerals and ammonia via transcellular and paracellular routes in the gastrointestinal (GI) tract influencing nutrient transporter gene expression and molecular structures of tight junctions. Some PSC stimulate nutrient absorption, while other PSC may impair transport mechanisms in the intestine interacting with nutrient transporters of enterocytes and tight junction motifs and their regulatory proteins. One challenging aspect is to select an effective dose at which a specific PSC could improve GI nutrient absorption while preserving or improving other beneficial biological effects. The optimum doses and precise molecular mechanisms for PSC are yet to be identified to understand discrepant observations among different studies and to improve the targeted biotechnological and pharmaceutical uses of PSC in farm animals. This review discusses the effects of different PSC on nutrient transport and permeability of GI epithelia and their mechanism of actions focusing mainly on livestock species.

 

Key words: Gastrointestinal tract, Ion channel, Nutrient absorption, Permeability, Plant secondary compound

 
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Review Article

BLOCKING  T-CELL  NEGATIVE  SIGNAL  REDUCE  TUMOR  BURDEN  TO  MINIMAL


----------------------------------------------------T. K.  GOSWAMI

Immunology Section

Indian Veterinary Research Institute

Izatnagar-243 122, U.P., India


Sustainable T-cell activation for tumor regression without inducing autoimmunity has been targeted by the cancer biologist. Alternatively blocking the T-cell negative signaling that has a retarding effect on T-cell activation signal may preclude tumor progression an unusual approach has been proposed recently. The proponent of the new concept James Patrick Allison working at US and Tasuku Honjo from Japan conceptualized that activation signal is similar like accelerator and negative signal is brake of a moving car, therefore releasing the brake may propel the vehicle forward without additional acceleration. Both Allison and Honjo has shared the Nobel Prize in Physiology and Medicine for the year 2018 for their original concept of blocking T-cell negative signal in cancer immunotherapy. Specific blocking of CTLA-4 and PD-1 two distinct protein molecules expressed on T-cell by Allison and Tasuku Honjo respectively using monoclonal antibody eventually leads to tumor regression in clinical cases.


Key words: Cancer, CTLA-4, Nobel Prize, PD-1, T-cell

 
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Research Article

TRANSCRIPTOME  ANALYSIS  TO  CHARACTERIZE  GENES  RELATED  TO  THE  MARBLING  TRAIT  ON  MEAT  QUALITY  OF  ABERDEEN  ANGUS


 -----------------------------------X. WANG, Y. ZHANG, D. WANG, G, JIN, B.LI, F. XU AND J. CHENG


Institute of Animal Husbandry and Veterinary

Shanxi Academy of Agricultural Sciences

Taiyuan, Shanxi, 030 032, China


We compared the intramuscular fat deposition in ribeye and subcutaneous fat muscle of Aberdeen Angus beef cattle. Eight samples of the best and worst muscle marbling grade of Angus cattle were collected from 120 Angus cattle after slaughtering. High-quality RNA was subsequently extracted from the beef ribeye and subcutaneous fat muscle and the transcriptome was sequenced using RNA-seq technology. Approximately 37.22 GByte of clean sequencing reads were obtained in this study. A total of 15 differentially expressed genes (DEGs) and 16 DEGs were detected in the ribeye and subcutaneous fat muscle tissues respectively. In the beef ribeye, six genes were up-regulated (CYP1A1, RRAD, RETRG1, LOC787269, XIRP1, OTUD1) and nine genes down-regulated (Bos_taurus_newGene_1359, Bos_taurus_newGene_4387, KRT18, MYL9, ACTA2, PTGDS, DSTN, CD5L, MYL6B), whereas the subcutaneous fat muscle, characterized by good marble meat quality, had 14 genes up-regulated (CYP1A1, SLC25A13, S100A1, KLHL40, THRSP, MYLK4, TNFRSF12A, RRAD, HMOX1, AMPD3, DNAJA4, PTPN3, LOC782922, IFITM10) and two down-regulated genes (Bos_taurus_newGene_996, FST). Gene analysis revealed that the differentially expressed genes were mostly associated with myocyte differentiation, for example MYL9, ACTA2 and MYLK4 genes. Gene ontology (GO) was then used to perform a term enrichment analysis and significant GO terms included the vascular smooth muscle contraction pathway and arachidonic acid (AA) metabolism. Taken together, we observed that the DEGs play an important role in marble texture and intramuscular fat deposition of the different parts of Angus cattle. Hence, a strong basis for improving the meat quality of Aberdeen Angus was considered.


Key words: Angus, DEGs, Intramuscular fat content, Marbling grade, Transcriptome

 
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Research Article

EFFECT  OF  SOYBEAN  LECITHIN  EXTENDER  ON POST-THAW  SEMEN  QUALITY OF  BENGAL  BUCK


----------------------------------------A. AKOURKI, M. MONDAL, M. KARUNAKARAN AND P. PAL


ICAR-National Dairy Research Institute

Eastern Regional Station

Kalyani-741 235, WB, India


Semen extenders containing lecithin of animal origin are frequently criticized because of the risk of disease dissemination. To look for some alternative extender, the present study was carried out with the aim to evaluate if egg yolk lecithin could be replaced by soybean lecithin by adding glycerol to the extender either at the temperature of 30°C or 5°C during cryopreservation of buck semen. For the purpose, ejaculates collected from four Bengal bucks by artificial vagina method were diluted and frozen either in a Tris-egg yolk or Tris-soybean lecithin with glycerol added either at 30°C or 4°C. Results revealed that sperm motility and plasma membrane and acrosome integrity were not affected (p>0.005) between the semen fraction frozen in an egg yolk and soybean lecithin based extenders. Moreover, adding glycerol to the extender at 30°C or 4°C did not change significantly (p>0.05) the quality of semen diluted and frozen in both extenders. Therefore, soybean lecithin can properly replace egg yolk lecithin during semen freezing of Bengal bucks and glycerol as a cryoprotector may be added to the extender either at 30°C or 4°C.


Key words: Buck, Freezing-thawing, Glycerol, Lecithin, Semen, Soybean

 
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Research Article

DETECTION OF LEPTOSPIROSIS AND LISTERIOSIS IN BOVINE ABORTION: AN IMMUNOHISTOCHEMICAL STUDY


---------------------------------------------------V. MAHAJAN, H. S. BANGA, G. FILIA AND K. GUPTA


 Guru Angad Dev Veterinary and Animal Sciences University

Ludhiana- 141 004, Punjab, India


 The present study was conducted to decipher Leptospira and Listeria associated abortion in bovine using immunohistochemical techniques. Immuno-histochemical staining for antigens of Leptospira interrogans in fetal kidney revealed positive staining of organisms preferentially in lining tubular lumen and within tubular epithelial cells. Listerial antigen was identified as principally intracellular (in neutrophils and macrophages) evident as deep brown, short rods and/or clusters of organism(s) within the abscesses and area of necrotic foci. Immuno-histochemical method can be used as a rapid tool in the diagnosis of Leptospira and Listeria abortion cases.


Key words: Abortion, Immunohistochemistry, Leptospirosis, Listeriosis

 
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Research Article

INVESTIGATION  OF  PESTE  DES  PETITS  RUMINANTS  (PPR)  OUTBREAK  AMONG  GOATS  AND  SHEEP  OF  AGASAVALLI  VILLAGE  (SHIVAMOGGA  DISTRICT,  KARNATAKA)


-------------------------------N. KARBASANAVAR, P. S. BAGALAKOTE, B. E. SHAMBULINGAPPA

K. J. ANANDA, S. P. SATHEESHA, B. KAVITHA RANI AND K. C. MALLINATH


Karnataka Veterinary, Animal and Fisheries Sciences University

Veterinary College, Shivamogga - 577 204

Karnataka, India


Peste des petits ruminants (PPR) is a highly contagious and economically important disease of small ruminants associated with high morbidity and mortality. Outbreak investigation was carried out in the month of August 2015 in goat and sheep of Agasavalli village (Shivamogga district, Karnataka). A total of 341 goats and 60 sheep reared under semi-intensive system were investigated for morbidity, mortality and case fatality. Affected animals exhibited symptoms suggestive of PPR viz. high fever, severe coughing, facial swelling, purulent nasal discharge, ulceration (gum, dental pad and oral commissure), profuse watery diarrhea with foul smell, anorexia and abortions in pregnant animals leading to death in about a week. Representative samples (n=10) collected from ailing animals were analysed in the laboratory and post-mortem examination of dead animals were carried out. Lateral flow assay (serum) and polymerase chain reaction (PCR) of nasal swabs confirmed PPR virus infection as the cause of outbreak among sheep and goats. Morbidity, mortality and case fatality due to PPR during the outbreak were 76.5%, 24.9% and 32.6% in goats and 55.0%, 5.0% and 9.1% in sheep, respectively. Severity of infection was higher among young animals compared to adults. Keeping in view high morbidity and mortality of PPR infection in small ruminants, proper prophylactic vaccination of small ruminants is advocated for the management of disease.


Key words: Goat, Lateral flow assay, Outbreak, PCR, Sheep

 
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Research Article

SEROPREVALENCE PATTERNS OF BOVINE BRUCELLOSIS IN ORGANISED AND UNORGANISEDFARMS OF COASTAL ANDHRA PRADESH, INDIA


---------------------------------------------------------B. DEEPTHI, R. N. RAMANI PUSHPA, M. SRIVANI, K. L. KAVITHA AND K. V. SUBRAMANYAM


Department of Veterinary Microbiology

NTR College of Veterinary Science, Gannavaram

Krishna Dt- 521 102, Andhra Pradesh, India


Brucellosis is an economically important infection of livestock and humans due to the reproductive problems it causes and also the risk to the public health. A study of bovine brucellosis was conducted using different serological tests to determine the disease status in an organized dairy farm and two private dairy farms in coastal area of Andhra Pradesh. A total of 445 serum samples from 200 cattle and 245 buffaloes were screened using Rose Bengal plate test (RBPT), Serum agglutination test (SAT), Lateral flow assay (LFA) and Indirect enzyme linked immunosorbent assay (i-ELISA). An overall prevalence of 9.88%, 7.78%, 6.29% and 7.86% was detected by RBPT, SAT, LFA and i-ELISA respectively. The present study identified overall high prevalence of bovine brucellosis in un-organized private farms particularly in female white cattle. RBPT and i-ELISA can be used successfully to declare the disease status of the herd and to implement control programmes like test and culling of the affected animals and adoption of strict hygienic measures in the farm.


Key words: Bovine Brucellosis, iELISA, RBPT, SAT, Screening

 
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Research Article

ULTRASONOGRAPHY  OF  LIVER  AND  SPLEEN  IN  BOVINE  BENIGN  THEILERIOSIS


-----------------------------------------------------N. DEVADEVI, K. RAJKUMAR AND P. VIJAYALAKSHMI


Department of Veterinary Medicine
Rajiv Gandhi Institute of Veterinary Education and Research
Pondicherry- 605 009, India


Ultrasonographic evaluation of liver and spleen was carried out in cattle suffering from bovine benign theileriosis. Diagnosis revealed bovine benign theileriosis (BBT) caused by Theileria orientalis and the conformation was done by Polymerase chain reaction. The ultrasonographic examination of the liver revealed increased echogenicity, which denotes diffuse hepatic changes, when correlated with the serum biochemistry showed a significant (P<0.05) reduction in mean serum albumin and  an alteration in albumin-globulin ratio. The mean serum total protein and mean globulin was significantly (P<0.01) increased in theileriosis affected cattle compared to that of control group. The mean creatinine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) had no significant difference in theileriosis cattle. The ultrasonography of splenic echogenicity was normal and within the parenchyma numerous tiny regularly spaced echoes and the blood vessels were imaged as anechoic round, oval or elongated images.


Key words: Diffuse hepatitis, Theileria orientalis, Utrasonography

 
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Research Article

AN OUTBREAK OF PASTEURELLOSIS IN JAPANESE QUAIL CHICKS (COTURNIX COTURNIX JAPONICA)


--------------------------------------N. BABU PRASATH, J. SELVARAJ, P. PONNUSAMY1 AND M. SASIKALA


Department of Veterinary Pathology

Veterinary College and Research Institute

TANUVAS, Orathanadu- 614 625, Tamilnadu, India


Avian pasteurellosis is a contagious disease of domestic and wild birds caused by Pasteurella multocida. A private broiler quail farm (n=1100) with a mortality of 330 eight day old quail chicks without clinical signs were presented to the Department of Veterinary Pathology, Veterinary College and Research Institute, Orathanadu, Tamilnadu, India. On complete post-mortem examination, visceral organs showed generalised congestion and consolidation of lung. Histopathologically, liver showed severe congestion, multifocal necrotic hepatitis with heterophilic infiltrations. Lungs revealed bronchopneumonia. Cytological examination of lung impression showed bipolar organisms. Microbiological examination with heart swab, lung and liver tissue revealed Pasteurella sp. The bacteria were confirmed by PCR assay with KMT1 gene as Pasteurella multocida. Based on history, gross, cytology, microbiological and histopathological lesions the case was diagnosed as avian pasteurellosis.


Key words: Japanese quail, Pasteurellosis, Pathology, Polymerase chain reaction

 
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Research Article

PREPARTUM  ADMINISTRATION  OF  VITAMIN E  AND  SELENIUM  INJECTION  AND  ITS  ABIDING  EFFECT  ON  CALF  WEIGHT,  PLACENTAL  WEIGHT  AND  EXPULSION  TIME  OF FETAL  MEMBRANE  IN  SURTI  BUFFALOES


---------------------------L. C. MODI, C. T. KHASATIYA, M. D. PATEL, K. K. TYAGI AND F. MODI


Department of Veterinary Gynaecology and Obstetrics

College of Veterinary Science and Animal Husbandry

Navsari Agricultural University, Navsari, Gujarat - 396 450, India


A study was conducted on twenty (20) Surti buffaloes during their transient period categorized into two groups; treatment (n=10) and control (n=10) groups. In treatment group of animals to which 10 mL DL-a tocopheryl acetate I.P. equivalent to tocopherol (vitamin E) base -50mg, sodium selenite U.S.P. equivalent to selenium base -1.5mg in each mL (E-CARE Se) and in control group animals 10 mL normal saline injected i/m on 60th, 45th, 30th and 15th day before expected date of parturition and after parturition on 15th and 30th day. The mean expulsion time of fetal membranes in the treatment group was found to be significantly shorter than that of control group. The mean placental weight in the treatment group was found to be non-significantly lower than that of control group. The calf weight in the treatment and control group did not differ significantly.


Key words: Buffaloes, Calf weight, Placental studies, Prepartum, Selenium, Vitamin E

 
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Research Article

CONSTRAINTS BEING PERCEIVED BY SHEEP REARERS IN ARID AND SEMIARID REGION OF RAJASTHAN


------------------------------------M. L. CHOUDHARY, VIJAY KUMAR, S. C. GOSWAMI, V. SINGH AND V. KUMAR


Department of Livestock Production Management

College of Veterinary and Animal Science

Rajasthan University of Veterinary and Animal Sciences

Bikaner -334 001, Rajasthan, India


The data were collected from 120 sheep rearers selected from 8 villages of two tehsils of Bikaner district. The information was collected from respondents on breeding practices of sheep by an interview schedule. On the basis of Rank based quotient (RBQ) value the main constraints identified were lack of grazing area and non-availability of green fodder with the RBQ values of 64.08 and 62.50 respectively. Lack of veterinary services was the third most serious constraint perceived by respondents obtaining RBQ value at 58.42%. Similarly, other constraints identified by respondents were ranked on the basis of the RBQ values.


Key words: Arid and semi arid region, Constraints, Respondents, Sheep rearers

 
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Research Article

PLASMA LEUTINIZING HORMONE PROFILING IN NORMAL AND REPEAT BREEDING KANKREJ  COW


--------------------------------------N. F. CHAUDHARI, B. N. SUTHAR, SANDHYA S. CHAUDHARI, C. F. CHAUDHARI AND L. C. MODI


Department of Veterinary Gynaecology and Obstetrics

College of Veterinary Science and Animal Husbandry

Navsari Agricultural University, Navsari

Gujarat - 396 450, India


The present investigation on plasma leutinizing hormone (LH) profile of 12 Kankrej cow (6 normal and 6 repeat breeding) was carried out on the day of estrus. The mean plasma LH concentration at 2 hours intervals from 8 hours to 36 hours post estrus was significantly (p<0.05) higher in control as compared to repeat breeder group at all the period of sampling except during first two collection where non-significant higher level was observed. Further, in control group the level of plasma LH was increased from 8 hrs. after onset of estrus and reached a peak level at 20 hours (4.62 ± 0.52 mIU/mL) and thereafter it declined. Whereas, no such trend was observed in repeat breeder group.


Key words: ELISA, Estrus, Kankrej cow, LH, Repeat breeding

 
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Research Article

EFFECTS OF PROPYLENE GLYCOL AND MAGNESIUM CHLORIDE AGAINST  DERMATOPHYTES ISOLATED  FROM COMPANION ANIMALS


-------------------------------------A. D. SINGH, C. DEBNATH, A. BANERJEE, K. BATABYAL, B. ROY AND I. SAMANTA


Department of Veterinary Public Health

Faculty of Veterinary and Animal Sciences

West Bengal University of Animal and Fishery Sciences

37, K. B. Sarani, Kolkata-700 037, W.B., India


The present study was undertaken to detect the occurrence of different dermatophytes in clinically suspected dogs and cats, and to evaluate the antifungal activities of propylene glycol and magnesium chloride against the isolates. A total of 517 samples including skin scrapings, hair, and nail scrapings were collected from suspected dogs (n=428) and cats (n=89) in West Bengal, India. Standard mycological technique was followed for isolation and identification of dermatophytes. Microsporum canis was isolated with highest frequency from both dogs and cats followed by Trichophyton mentagrophytes and Microsporum gypseum. Minimum inhibitory concentration of propylene glycol against selected isolates of M. canis, M. gypseum and T. mentagrophytes was detected as 0.008 g /L, whereas, magnesium chloride produced a minimum inhibitory concentration of 0.016 g /L against the same isolates. The study confirmed antifungal effect of both propylene glycol and magnesium chloride against studied dermatophyte isolates and better inhibitory property of propylene glycol.


Key words: Antifungal, Dermatophyte, Magnesium chloride, Propylene glycol

 
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Short Communication

SEMEN CHARACTERISTICS IN YOUNG AND ADULT  SURTI  BUCK


-------------------------------------V. B. ATARA, C. F. CHAUDHARI, U. V. RAMANI, M. M. CHAUDHARY, D. K. PATEL, Y. R. PATEL AND  N. G. PATEL


Department of Veterinary Gynaecology and Obstetrics, Veterinary College

Navsari Agricultural University, Navsari, Gujarat- 396 450, India


Present study was conducted to study the effect of age on semen parameters in Surti buck. Age was significantly influenced on majority of the semen parameters in Surti bucks. Adult bucks significantly (P<0.01) performed better in terms of semen quantitative parameters viz., semen density (3.91+0.03 vs 3.79+0.03), volume (1.01+0.03 vs 0.86+0.03 mL), sperm concentration (2564.22+87.27 vs 1978.13+60.79 million/mL), total sperm count (2483.83+115.22 vs 1546.93+62.96 million) and semen index (1737.19+78.10 vs 1104.39+47.29 million). However, semen qualitative parameters viz., initial progressive motility (93.75+0.22 vs 93.72+0.21%), live sperm count (75.36+0.93 vs 75.93+0.74%), sperms with physically intact plasma membrane (85.28+0.27 vs 85.31+0.19%), progressive motility at various intervals and morphologically normal spermatozoa were at par in adult as well as in young Surti bucks.


Key words: AV method, Semen ejaculates, Semen parameters, Surti buck

 
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Short Communication

MANAGEMENT OF POST-PARTUM UTERINEPROLAPSE IN A GOAT WITH VULVAL LIPSUTURE TECHNIQUE


-----------------------------------K. P. SINGH, B. SINGH1, R.V. SINGH, P. SINGH AND S. K. SINGH


Government Veterinary Hospital

Department of Animal Husbandry

Deoranian, Bareilly,Uttar Pradesh-243 203, India


The present study was conducted for management of post-partum uterine prolapse in a non-descript goat of 25 Kg body weight with vulval lip suture technique. A two years old goat of 2nd pregnancy was presented with history of uterine prolapse following normal kidding of 2 kids 48 hours earlier. The prolapsed uterus was injured, swollen, edematous and contaminated with dung and wheat straw. The prolapsed mass was lacerated and had numerous bleeding points. Vaginal wall was edematous, swollen, thicker and bluish pink in appearance. The epidural anesthesia was achieved by giving 2% lignocaine hydrochloride @ 4 mg/ Kg body weight into lumbosacral space to prevent straining during replacement of prolapsed mass. The prolapsed mass was washed with chilled water containing potasium permagnate (1: 1000) followed by flushing with metronidazole solution. The prolapsed mass was lubricated with xylocain gel and replaced into normal position by gentle pressure. The body of the uterus was first pushed forward followed by horns. Buhner suture using nylon (size 0) was placed in the vulva as a retention technique to hold the uterus in place. The animal was treated with injection calcium magnesium borogluconate @ 1mL/ Kg body weight, 5 % dextrose normal saline (DNS), oxytocin. ceftriaxone, meloxicam, chlorphenramine maleate and B1, B6 and B12. The owner was also advised to apply antimicrobial ointment on suture line for preventing infection. The animal showed recovery after 3 days and further no prolapse was reported by the owner. The Buhner suture was removed after 7 days post management.


Key words: Epidural anesthesia, Prolapsed mass, Reduction, Reposition, Retention

 
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Short Communication

SEROPREVALENCE  OF  CHLAMYDOPHILA   INFECTION  IN  SHEEP  OF  KRISHNA  DISTRICT,  ANDHRA  PRADESH


----------------------------G. DEEPIKA KUMARI, R. N. RAMANI PUSHPA  AND B. KEERTHI


Department of Veterinary Microbiology

NTR College of Veterinary Sciences

Gannavaram, Andhra Pradesh- 521 102, India


The present study was aimed to assess the seroprevalence of chlamydial infection in sheep. Chlamydophila abortus infection is characterized by reproductive losses in livestock posing heavy economic losses to farmers. Occurrence of the disease is high during the lambing season and C. abortus efficiently colonizes in the placental trophoblasts causing enzootic abortions. Moreover, it represents a zoonotic risk to humans who come in contact with aborted placental material. The present sero-prervalence study was conducted to identify the presence of chlamydial infection in sheep. A total of 28 serum samples were collected from the aborted cases in Krishna district of Andhra Pradesh. The seroprevalence of chlamydial infection was studied by enzyme linked immonosorbent assay (ELISA) for detection of antibodies in the serum samples of suspected chlamydial infection. Out of 28 serum samples tested, four samples were positive for the Chlamydophila abortus infection in sheep. The percentage of Chlamydophila abortus infection in sheep was 14. The present study indicated the prevalence of bacterial pathogen Chlamydophila abortus infection as a cause of abortions in sheep.


Key words: Andhra Pradesh, Chlamydophila abortus, Enzootic abortions, Ruminants, Seroprevalence

 
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Short Communication

UTERINE LEIOMYOMA IN A COW –A CASE REPORT


---------------------------------------V. SACHAN, R. KUMAR, R. K. GUPTA, A. SAXENA


Department of Animal Reproduction, Gynecology and Obstetrics

College of Veterinary Sciences and Animal Husbandry

Mathura-281 001, Uttar Pradesh, India


A cow was reported with the difficulty in parturition since last 12 to 15 hours. Investigation of the birth canal revealed that the cause of dystocia was obstruction, due to large lump at or near the internal os. After relieving the dystocia, it was found that the lump, may be a neoplastic mass, was confirmed as leiomyoma after histopathology.


Key words: Cow, Dystocia, Leiomyoma, Neoplasia

 
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Short Communication

ALTERATIONS IN SERUM AST, ALT AND ALKALINE PHOSPHATASE ACTIVITY DURING DIFFERENT DAYS OF FMD INFECTION IN LACTATING COWS


---------------------------------T. K. SAR, R. BURAGOHAIN, S. AKHTAR AND T. K. MANDAL


Department of Veterinary Pharmacology and Toxicology

West Bengal University of Animal and Fishery Sciences

37, K.B.Sarani, Kolkata – 700 037, India


The present study aims monitoring of AST, ALT and alkaline phosphatase activity on different days in six healthy and six FMD affected lactating cows in field condition. The FMD affected six lactating cows were selected on the basis of clinical signs including high temperature, excessive salivation, formation of vesicles on the oral and nasal mucosa, the inter-digital spaces and coronary bands on the feet. Six apparently healthy lactating cows in the field condition were considered as control group. One mL of blood was collected aseptically from each cow from jugular vein at day 0, 3, 6, 12 and 21. AST, ALT and alkaline phosphatase activity were estimated as per reported methods. AST activity was found to be increased significantly (p<0.05) on 0, 3 and 6 day in FMD affected cows compared to healthy cows. Alkaline phosphatase activity was increased significantly (p<0.05) on 0, 3, 6 and 12 day in FMD affected cows compared to healthy cows indicating liver tissue damage. However, the alkaline phosphatase activity was observed to return to the normal level on day 21 in FMD affected cows without systemic application of any drug. Interestingly, ALT activity did not alter significantly in FMD affected cows compared to healthy cows. The observations may be correlated with liver impairment in FMD affected cows.


Key words: Alkaline phosphatase, ALT, AST, Cows, FMD

 
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Short Communication

CERVICO-VAGINAL PROLAPSE AND ITS MANAGEMENT: A CASE REPORT


-----------------------------V. VAID, T. GUPTA, K. MAHAJAN, S. F. AHMAD AND A. SINGH


Faculty of Veterinary Sciences and Animal Husbandry

Sher-e-Kashmir University of Agricultural Sciences and Technology- Jammu

Jammu and Kashmir, India-180 009


Four years old cattle in its first month of pregnancy was presented with  complaint of a mass hanging from the genital opening. Its recurrence was reported by the owner. General examination of the animal was carried out and the temperature was recorded as 99.8o F. The animal exhibited the signs of restlessness and the protruding mass was edematous and swollen. The physical examination confirmed cervico-vaginal prolapse. The perineal region of the cow was prepared for aseptic surgery. The animal was operated under epidural anesthesia using 2% lignocaine hydrochloride (10 mL). The prolapsed mass was cleaned with dilute (1%) potassium permanganate solution and the mass was lifted with both hands and replaced into the vagina by using fist support. Afterwards, 2% lignocaine hydrochloride was administered subcutaneously into the lips of the vagina where the Gerlach needle was punctured. Buhner’s suture was applied with rope parallel to the vulva keeping one hand space for easy urination. Sutures were removed after 1 month of the surgical intervention. The owner was advised for proper post-operative care for preventing its recurrence. The cow was followed for the next 2 months. It recovered successfully without any further complications.


Key words: Buhner’s suture, Cervico-vaginal prolapse, Gerlach needle, Tenesmus

 
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Short Communication

MANAGEMENT OF HYDRALLANTOIS IN A NON-DESCRIPT COW BY TRANSCERVICAL ALLANTOCENTESIS

---------------------------------------A. KUMAR, A. SAXENA, K. K. MISHRA AND R. ASRE


Institute of Para-Veterinary Science

DUVASU, Mathura- 281 001

Uttar Pradesh, India


Sudden, abnormal accumulation of allantoic fluid in allanto-chorionic sac pertains hydrallantois. The aforesaid condition can be treated by caesarean section or slow drainage of fetal fluid to avoid hypovolemia. Present case uses transcervical allantocentesis method along with other regular medicines for management of hydrallantois.


Key words: Cow, Gestational disorder, Hydrallantois, Placenta, Pregnancy

 
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