Abstract

The present study was aimed to detect PPRV in clinical samples using S-ELISA, N & F gene based reverse transcriptase PCR (RT-PCR) and cell culture techniques. A total of 119 clinical samples comprised of nasal (62), oral (16) and conjunctival swabs (20) and tissues (21) were collected from goats (95) and sheep (24) from different districts of Saurashtra region of Gujarat for detection of PPRV. Thirty-one animals were found positive for PPRV by all four tests. Two samples were found negative by N gene-based RT-PCR but positive by S-ELISA. Relative to S-ELISA, sensitivity, specificity, and overall agreement for N gene-based RT-PCR was 94.59%, 100% and 98.32%, respectively, while for F gene-based RT-PCR, it was 86.49%, 100% and 95.80%, respectively. Representative clinical samples positive for PPRV by S-ELISA (37) were subjected to isolation and propagation of PPR virus in Vero cells. Out of 37 S-ELISA positive samples, 31 samples showed CPE on first passage, which was further confirmed by RT-PCR.

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